Neuromuscular and Skeletal Diseases Working Group
Key research areas
The Neuromuscular and Skeletal Diseases working group, led by Univ.-Prof. Brunhilde Wirth, PhD, has been working since 1990 on the elucidation of genetic and pathomechanical causes of neuromuscular diseases, in particular spinal muscular atrophies (SMA) and diseases of the second motor neuron in the spinal cord. Recently, the group has also focused on osteoporosis and bone development. We are using state-of-the-art methods of exome and genome sequencing, transcriptome analysis, genome editing and induced pluripotent cells to identify genetic causes. Over the years, our laboratory has built up a wide range of methods to understand biochemical, cellular and pathological changes in motor neuron diseases and osteoporosis. We develop conditional and transgenic mouse models, zebra fish models and, more recently, Drosophila models for detailed analyses. Ultimately, this knowledge will contribute to the development of therapies, which is a particular focus of the laboratory.
A. Molecular genetics and biology of spinal muscular atrophy - elucidating the pathogenesis and searching for therapy
- Search for SMA-modifying factors
- Molecular biology and functional investigations of the survival motor neuron (SMN) genes and SMN interacting proteins to reveal cellular malfunctions of SMA
- Investigating alternative splicing of SMN2
- Developing a therapeutic approach for SMA patients based on activation of the SMN2 gene and the influencing SMA-modifying factors
B. Identifying new causal genes and functional characterization of unexplained motor neuron diseases
- Recruiting families and patients with motor neuron diseases
- Excluding known genes by sequencing all known genes on a motor neuron gene panel
- Exome sequencing to identify underlying pathogenic variants
- Functional analysis of potential pathogenic variants in cellular systems and model organisms
C. Osteoporosis: Revealing molecular causes of osteoporosis in PLS3 loss
- Analysing the function of PLS3 in the development of bones in transgenic mice that overexpress PLS3
- Analysing the impact of PLS3 overexpression on other bone diseases
- Analysing PLS3 loss in conditional knockout mice
- Clarifying the underlying pathological mechanism of osteoporosis due to PLS3 loss
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) (Wi 945/17-1; Wi945/18-1; Wi 945/19-1; RTG1960; FOR2722)
- CMMC (C16)
- SMA Europe
- Cure SMA
- EU-HORIZON 2020
- SMABEYOND
Torres-Benito L., Schneider S., Rombo R., Ling K.K., Grysko V., Upadhyay A., Kononenko N.L., Rigo F., Bennett C.F. & Wirth B.
NCALD Antisense Oligonucleotide Therapy in Addition to Nusinersen further Ameliorates Spinal Muscular Atrophy in Mice.
Am J Hum Genet. 2019 Jul 3; 105(1):221-230
PubMed PMID: 31230718
Janzen E, Mendoza-Ferreira N, Hosseinibarkooie S, Schneider S, Hupperich K, Tschanz T, Grysko V, Riessland M, Hammerschmidt M, Rigo F, Bennett CF, Kye MJ, Torres-Benito L, Wirth B.
CHP1 reduction ameliorates spinal muscular atrophy pathology by restoring calcineurin activity and endocytosis.
Brain. 2018 Aug 1;141(8):2343-2361
PubMed PMID: 29961886
Riessland M*, Kaczmarek A*, Schneider S*, Swoboda KJ, Löhr H, Bradler C, Grysko V, Dimitriadi M, Hosseinibarkooie S, Torres-Benito L, Peters M, Upadhyay A, Biglari N, Kröber S, Hölker I, Garbes L, Gilissen C, Hoischen A, Nürnberg G, Nürnberg P, Walter M, Rigo F, Bennett CF, Kye MJ, Hart AC, Hammerschmidt M, Kloppenburg P, Wirth B.
Neurocalcin Delta Suppression Protects against Spinal Muscular Atrophy in Humans and across Species by Restoring Impaired Endocytosis.
Am J Hum Genet. 2017;100(2):297-315.
PubMed PMID: 28132687
Hosseinibarkooie S*, Peters M*, Torres-Benito L, Rastetter RH, Hupperich K, Hoffmann A, Mendoza-Ferreira N, Kaczmarek A, Janzen E, Milbradt J, Lamkemeyer T, Rigo F, Bennett CF, Guschlbauer C, Büschges A, Hammerschmidt M, Riessland M, Kye MJ, Clemen CS, Wirth B.
The Power of Human Protective Modifiers: PLS3 and CORO1C Unravel Impaired Endocytosis in Spinal Muscular Atrophy and Rescue SMA Phenotype.
Am J Hum Genet. 2016 Sep 1;99(3):647-65.
PubMed PMID: 27499521
van Dijk FS, Zillikens MC, Micha D, Riessland M, Marcelis CL, de Die-Smulders CE, Milbradt J, Franken AA, Harsevoort AJ, Lichtenbelt KD, Pruijs HE, Rubio-Gozalbo ME, Zwertbroek R, Moutaouakil Y, Egthuijsen J, Hammerschmidt M, Bijman R, Semeins CM, Bakker AD, Everts V, Klein-Nulend J, Campos-Obando N, Hofman A, te Meerman GJ, Verkerk AJ, Uitterlinden AG, Maugeri A, Sistermans EA, Waisfisz Q, Meijers-Heijboer H, Wirth B, Simon ME, Pals G.
PLS3 mutations in X-linked osteoporosis with fractures.
N Engl J Med. 2013 Oct 17;369(16):1529-36. doi: 10.1056/NEJMoa1308223. Epub 2013 Oct 2.
PubMed PMID: 24088043
Oprea GE, Kröber S, McWhorter ML, Rossoll W, Müller S, Krawczak M, Bassell GJ, Beattie CE, Wirth B
.
Plastin 3 is a protective modifier of autosomal recessive spinal muscular atrophy.
Science. 2008;320(5875):524-7
PubMed PMID: 18440926
Brichta L, Hofmann Y, Hahnen E, Siebzehnrubl FA, Raschke H, Blumcke I, Eyupoglu IY, Wirth B.
Valproic acid increases the SMN2 protein level: a well-known drug as a potential therapy for spinal muscular atrophy.
Hum Mol Genet, 2003;12:2481-9.
PubMed PMID: 12915451
Feldkötter M, Schwarzer V, Wirth R, Wienker TF, Wirth B.
Quantitative analysis of SMN1 and SMN2 based on real-time LightCycler PCR: Fast and highly reliable heterozygosity testing and prediction of severity of spinal muscular atrophy.
Am J Hum Genet, 2002, 70:358-368.
PubMed PMID: 11791208
Lorson CL*, Hahnen E*, Androphy EJ, Wirth B.
A single nucleotide in the SMN gene regulates splicing and is responsible for spinal muscular atrophy.
Proc Natl Acad Sci USA, 1999, 96 (11):6307-6311
PubMed PMID: 10339583
B. Wirth & M. Riessland, US 61/859,664, Neurocalcin Delta Inhibitors and Therapeutic and Non-Therapeutic Uses Thereof, Patent filed 29.07.2014, Patent issued in US & EU in 2018
B. Wirth, E. Janzen, N. Mendoza-Ferreira, SM Hosseinibarkooie, EP 17172826, Calcineurin B Homologous Protein 1 Inhibitors and Therapeutic and Non-Therapeutic Uses Thereof, Patent filed 24.05. 2017, PCT/EP2018/063562
| Academic career | |
| 2015 | European Clinical Laboratory Geneticist |
| 1996 | Specialisation in human genetics at the Institute of Human Genetics, University of Bonn |
| 1996 | Post-doctoral professorship and research qualification at the Institute for Human Genetics at the University of Bonn (Prof Propping) |
| 1989 | Dissertation (PhD/Dr. rer. nat.) at the University of Bonn (Prof A. Gal) |
| 1982 | Master’s thesis in the Department for Cell Biology at the Faculty of Biology at the University of Bucharest, Romania (Prof Ionescu-Varo) |
| 1982–1983 | Specialisation in “applied genetics” at the Pasteur Institute of the University of Bucharest (Prof. Lungenau) |
| 1978–1982 | Biology degree at the University of Bucharest, Romania |
| Scientific and clinical history | |
| Since 2003 | Professor (C4) and Director of the Institute of Human Genetics at the University of Cologne, Head of the “Neuromuscular and Skeletal Diseases” working group |
| 1996–2003 | Senior research assistant (C2) at the Institute of Human Genetics at the University of Bonn, group leader |
| 1990–1996 | Research assistant (C1) at the Institute of Human Genetics at the University of Bonn, group leader |
| 1989–1990 | DFG post-doctoral student at the Imperial Cancer Research Fund Laboratorien in London (Prof Frischauf) |
| 1986–1989 | Doctoral student at the Institute of Human Genetics at the Medical University of Bonn (Prof Gal) |
| 1983–1985 | Biology teacher in Turnu Rosu, Romania |
| Awards and distinctions | |
| 2019 | NRW Innovation Award |
| 2001 | 1st Place Research Award “Motor-neuron Diseases” (Aventis, DGM) |
| 1996 | Sanofi-Wintrop Myopathy Award (DGM) |
| Memberships and service | |
| Seit 2016 | Chair of the Scientific Review Board of SMA Europe |
| Seit 2016 | Chair of the Max-Delbrück Future Award at the University of Cologne |
| 2010–2013 | Chair of the Review Board of the CMMC (Center for Molecular Medicine Cologne) |
| Seit 2009 | Member of the Scientific Review Board of the CMMC |
| 2009–2016 | Member of the Scientific Committee of the International Congress of Human Genetics |
| 2009–2016 | Chair of the Scientific Committee of the European Society of Human Genetics |
| Seit 2008 | Co-director of the European School of Medical Genetics |
| 2003–2017 | Member of the Scientific Committee of the European Society of Human Genetics |
| 2002–2006 | Vice President of the German Society for Human Medicine |
| Publications |
| > 160 publications (H factor: 61, >13,500 citations according to Google Scholar: as of 01/12/2019 |
Team
Andrea Delle Vedove, physician, MD-PhD candidate
Nur Cengiz, M.Sc., PhD candidate
Irmgard Hölker, medical-technical assistant
Dr. Mert Karakaya, clinical scientist, resident physician
Natalie Keller, MD candidate
Dr. Marlen Lauffer, M.Sc., clinical scientist, resident physician
Anixa Muinos-Bühl, M.Sc., PhD candidate
Ilka Müller, M.Sc., PhD candidate
Roman Rombo, B.Sc., biological-technical assistant
Bryony Charlotte Ross, physician, MD-PhD candidate
Tamas Schmidt, M.Sc., PhD candidate
Eike Strathmann, M.Sc., PhD candidate
Max Thelen, M.Sc., PhD candidate
Dr. rer. nat. Laura Torres-Benito, M.Sc., postdoctor
Lisa Wolff, M.Sc., PhD candidate
Sebastian Zetzsche, biological-technical assistant